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Research Title: Antinociceptive action of Ononis spinosa leaf extract in mouse pain models.
Author: Sahar Majdi Jaffal, Published Year: 2019
Acta Poloniae Pharmaceutica - Drug Research , 76(2): 299-304.
Faculty: Science

Abstract: Abstract: Ononis spinosa (spiny restharrow) is a perennial herb that belongs to the Fabaceae family. Among several uses, the aerial parts of O. spinosa are used in traditional medicine for toothache. In this study, analgesic activity of the methanolic extract of leaves was studied. In writhing test, the 300 mg/kg and 600 mg/kg doses of O. spinosa extract produced 90.1% and 94.5% inhibition of abdominal cramps, respectively compared to 23.6% inhibition produced by 30 mg/kg diclofenac sodium. O. spinosa methanolic extract reduced the time of licking in early and late phases of the formalin test. The inhibitory effect of O. spinosa extract was more pronounced in late phase. The lowest dose tested (300 mg/kg) of O. spinosa extract produced 81.0% inhibition compared to 50.5% inhibition in 30 mg/kg diclofenac sodium in the late phase of the formalin test. This effect was not abrogated by glibenclamide, naloxone or atropine indicating that the action of O. spinosa in formalin test is not mediated by ATP-sensitive K+ channel, opioid or muscarinic receptors. In tail-flick and hot-plate tests, O. spinosa increased the latency time at 30 min and 150 min. Naloxone antagonized the action of O. spinosa in both tests suggesting an interaction with the opioid receptor as a possible mechanism of O. spinosa in thermal pain models at the spinal and supraspinal levels. Phytochemical screening indicated the presence of phenolics, saponins, flavonoids, and terpenoids. Alkaloids, sterols, and anthraquinone glycosides were absent. HPLC analysis confirmed the presence of kaempferol, apigenin and myricetin in the extract.

Keywords: Ononis spinosa, analgesic activity, opioid receptor

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Research Title: Antinociceptive action of Achillea biebersteinii methanolic flower extract is mediated by interaction with cholinergic receptor in mouse pain models.
Author: Sahar Majdi Jaffal, Published Year: 2019
Inflammopharmacology , 7(5): 961-968.
Faculty: Science

Abstract: Achillea biebersteinii is a perennial aromatic herb that grows in the Mediterranean area. The leaves of this plant are used in foods as bittering and appetizing agents. In folk medicine, it is used for the treatment of stomachache and abdominal pain. In this study, the analgesic effect of A. biebersteinii methanolic flower extract was tested in three pain models, namely: writhing, tail-flick and paw-licking (formalin) tests. A. biebersteinii extract inhibited abdominal cramps produced by acetic acid. The effect of A. biebersteinii was better than that of 70 mg/kg indomethacin. In tail flick, A. biebersteinii extract increased latency at 30 min and was as effective as 100 mg/kg diclofenac sodium. In formalin test, A. biebersteinii extracts decreased paw-licking and flinching response in early and late phases. Atropine blocked the action of A. biebersteinii extract (300 mg/kg) in the late phase of formalin test as well as in writhing and tail-flick tests. GC-MS analysis revealed that ascaridole and iso-ascaridole were the main constituents of A. biebersteinii flower extract. In conclusion, this study shows for the first time that the antinociceptive effect of A. biebersteinii is mediated by the cholinergic receptor.

Keywords: Achillea biebersteinii; Antinociceptive effect; Cholinergic receptor; GC–MS; Paw-licking test; Tail-flick test; Writhing test.

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Research Title: Antinociceptive Action of Moringa peregrina is Mediated by Interaction with α2-Adrenergic Receptor.
Author: Sahar Majdi Jaffal, Published Year: 2020
Balkan Medical Journal , 37: 189-195.
Faculty: Science

Abstract: Background: Moringa peregrina (M. peregrina) is an edible, drought-resistant tree that is native to semi-arid countries. It is used as a painkiller in folk medicine. Aims: To study the antinociceptive effects of the leaf extract of M. peregrina in mice. Study Design: Animal experimentation. Methods: We employed thermal (hot plate and tail-immersion tests) and chemical (writhing and formalin tests) pain models in male BALB/c mice (eight animals per group) to investigate the mechanisms involved in the antinociceptive actions of M. peregrina. Additionally, we identified the chemical constituents present in the extract of M. peregrina by using liquid chromatography-mass spectrometry analysis, and predicted the possible active constituents that interact with the receptor based on molecular docking simulations. Results: In the writhing test, 200 mg/kg of M. peregrina extract restricted abdominal cramps by up to 55.97% (p<0.001). Further, it reduced the time of paw-licking in the early and late phases of formalin test by up to 56.8% and 65.5%, respectively, as compared to the percentage inhibitions of 50.5% and 48.4% produced by 30 mg/kg diclofenac sodium in the early and late phases, respectively (p<0.05). This effect was abrogated by yohimbine (1 mg/kg, intraperitoneally), but not by methysergide (5 mg/kg, intraperitoneally), in the late phase only, which indicates that the action of M. peregrina in formalin test is not mediated by 5-HT2 serotonin receptors, but rather via α2-adrenergic receptors. In the hot plate test, but not on tail-immersion test, the high dose (400 mg/kg) of the extract increased the latency time after 30 minutes of its administration. Yohimbine antagonized the action of M. peregrina in the hot plate test. Based on LC-MS analysis, the major constituents found in M. peregrina methanolic extract were chrysoeriol 7-O-diglucoside, lupeol acetate, quercetin, and rutin. Depending on the molecular docking results, the activity of M. peregrina extract could be due to the binding of chrysoeriol 7-O-diglucoside, quercetin, and rutin to the α2-adrenergic receptor. Conclusion: Interaction with the α2-adrenergic receptor serves as a possible mechanism of the M. peregrina analgesic effect.

Keywords: Adrenergic alpha-2 receptor antagonists, analgesics, molecular docking, Moringa peregrina

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Research Title: Anti-nociceptive effect of Arbutus andrachne L. methanolic leaf extract mediated by CB1, TRPV1 and PPARs in mouse pain models.
Author: Sahar Majdi Jaffal, Published Year: 2020
Inflammopharmacology 37:189-195.,
Faculty: Science

Abstract: Arbutus andrachne L. is a medicinal plant that grows in Jordan and has many valuable effects. In the present study, the anti-nociceptive effect of A. andrachne methanolic leaf extract was determined in mice using thermal and chemical tests. Our findings show that different doses of A. andrachne extract reduced the number of writhings significantly compared to control group. The leaf extract also reduced the time of paw licking in the early and late phases of formalin test. In all the conducted tests, 300 mg/kg body wt. was the best effective dose. A peroxisome proliferator-activated receptor alpha (PPARα) antagonist reversed the action of the plant extract in the early phase of formalin test while antagonists of the PPARα, PPAR gamma (PPARγ) and cannabinoid 1 (CB1) receptors were responsible for abolishing its effect in the late phase of this test. Also, the extract administration increased the latency time in hot plate and tail flick, an effect that was reversed by the antagonists of PPARγ, CB1 and transient receptor potential vanilloid 1 (TRPV1). No effect was noticed for α2-adrenergic receptor antagonist in the action of A. andrachne in any of the conducted tests in this study. Furthermore, analysis of the constituents in the methanolic leaf extract using liquid chromatography mass spectrometry (LCMS) showed that the extract is rich in compounds that have anti-nociceptive and/or anti-inflammatory effects such as arbutin, rutin, linalool, linoleic acid, gallic acid, lauric acid, myristic acid, hydroquinone, β-sitosterol, ursolic acid, isoquercetin, quercetin, (+)-gallocatechin, kaempferol, α-tocopherol, myricetin 3-O-rhamnoside and catechin gallate. In conclusion, A. andrachne showed promising anti-nociceptive effects in thermal and chemical models of pain. These findings can open an avenue for natural pain relief.

Keywords: Analgesia; Arbutus andrachne; CB1; PPAR; TRPV1; α2 adrenergic.

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Research Title: The desensitization of the transient receptor potential vanilloid 1 by nonpungent agonists and its resensitization by bradykinin.
Author: Sahar Majdi Jaffal, Published Year: 2020
Neuroreport , 31(11):781-786.
Faculty: Science

Abstract: Transient receptor potential vanilloid type-1 (TRPV1) channels have crucial roles in inflammatory hyperalgesia. Different inflammatory mediators can modulate TRPV1 sensitization. Bradykinin is an algogenic substance released at the site of inflammation. The aim of the present study is to investigate the desensitization of TRPV1 receptor by nonpungent agonists and to determine how bradykinin and prostaglandin E2 receptors (EP3 and EP4) modulate the resensitization of TRPV1 receptor after being desensitized by nonpungent agonists. Tail flick test was used to investigate capsaicin-induced thermal hyperalgesia and the desensitization of TRPV1 by the nonpungent agonists (olvanil and arvanil) in male BALB/c mice weighed (22-25 g). Resensitization of TRPV1 by bradykinin and the role of prostaglandin receptors in mediating sensitization of TRPV1 were also investigated. Intraplantar injection of capsaicin (0.3 µg) produced a robust thermal hyperalgesia in mice, while olvanil (0.3 µg) or arvanil (0.3 µg) produced no hyperalgesia, emphasizing their lack of pungency. Olvanil and arvanil significantly attenuated capsaicin-induced thermal hyperalgesia in mice. Bradykinin significantly reversed the desensitizing effects of arvanil, but not olvanil. EP4 but not EP3 receptors mediate the sensitization of TRPV1 By bradykinin in vivo. The present study provides evidence for a novel signaling pathway through which bradykinin can regulate the TRPV1 ion channel function via EP4 receptor.

Keywords: TRPV1, pain, EP3, EP4, hyperalgesia

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Research Title: Analgesic property of Loranthus acaciae studied by molecular docking and biological assays.
Author: Sahar Majdi Jaffal, Published Year: 2021
Systematic Reviews in Pharmacy, 12(03):712-719.
Faculty: Science

Abstract: Loranthus acaciae (Loranthaceae) is a perennial semiparasitic mistletoe. This study investigates the analgesic, antidepressant and anxiolytic effects of this plant. Different animal models were used. Also, chemical composition of the extract was analyzed using LC-MS. In writhing test, 150 and 300 mg/kg of L. acaciae inhibited abdominal cramps by 94.14 % and 94.78 %, respectively compared to 57.95 % inhibition produced by 70 mg/kg indomethacin. In hot-plate but not tail-flick test, 300 mg/kg L. acaciae significantly increased latency time. In formalin test, 300 mg/kg L. acaciae decreased paw-licking and flinching in early and late phases of formalin test by 68.5% and 83.3%, respectively compared to 39.2% and 56.1% inhibition by indomethacin (50 mg/kg). Naloxone and caffeine but not glibenclamide reversed the analgesic action of this plant in late phase while only caffeine reversed its action in early phase. In forced swimming and elevated plus maze tests, no statistically significant difference was found between L. acaciae-treated mice and control group. In open field test, L. acaciae decreased the number of lines crossed and rearing behavior. LC-MS analysis of the methanolic leaf extract identified 17 compounds. Loranthin was the major constituent. Based on the results of molecular docking, the activity of L. acaciae could be due to the binding of lupeol, campesterol & rhoifolin to μ-opioid receptor. This study indicates that the analgesic action of L. acaciae was mediated by interaction with opioid and adenosine receptors. No antidepressant or anxiolytic effects were exerted by L. acaciae.

Keywords: Loranthus acaciae, analgesia, pain, medicinal plants

47
Research Title: Ononis spinosa Alleviated Capsaicin-Induced Mechanical Allodynia in a Rat Model through TRPV1 Modulation.
Author: Sahar Majdi Jaffal, Published Year: 2021
Korean Journal of pain. , 34 (3):1-9.
Faculty: Science

Abstract: Background Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel implicated in pain sensation in response to heat, protons, and capsaicin (CAPS). It is well established that TRPV1 is involved in mechanical allodynia. This study investigates the effect of Ononis spinosa (Fabaceae) in CAPS-induced mechanical allodynia and its mechanism of action. Methods Mechanical allodynia was induced by the intraplantar (ipl) injection of 40 µg CAPS into the left hind paw of male Wistar rats. Animals received an ipl injection of 100 µg O. spinosa methanolic leaf extract or 2.5% diclofenac sodium 20 minutes before CAPS injection. Paw withdrawal threshold (PWT) was measured using von Frey filament 30, 90, and 150 minutes after CAPS injection. A molecular docking tool, AutoDock 4.2, was used to study the binding energies and intermolecular interactions between O. spinosa constituents and TRPV1 receptor. Results The ipsilateral ipl injection of O. spinosa before CAPS injection increased PWT in rats at all time points. O. spinosa decreased mechanical allodynia by 5.35-fold compared to a 3.59-fold decrease produced by diclofenac sodium. The ipsilateral pretreatment with TRPV1 antagonist (300 µg 4-[3-Chloro-2-pyridinyl]-N-[4-[1,1-dimethylethyl] phenyl]-1-piperazinecarboxamide [BCTC]) as well as the β2-adrenoreceptor antagonist (150 µg butoxamine) attenuated the action of O. spinosa. Depending on molecular docking results, the activity of the extract could be attributed to the bindings of campesterol, stigmasterol, and ononin compounds to TRPV1. Conclusions O. spinosa alleviated CAPS-induced mechanical allodynia through 2 mechanisms the direct modulation of TRPV1 and the involvement of β2 adrenoreceptor signaling.

Keywords: Butoxamine, Capsaicin, Fabaceae, Hyperalgesia, Molecular Docking Simulation, Neuralgia, Ononis, Pain, Stigmasterol, TRPV Cation Channels

48
Research Title: Ononis spinosa Alleviated Capsaicin-Induced Mechanical Allodynia in a Rat Model through TRPV1 Modulation.
Author: Sahar Majdi Jaffal, Published Year: 2021
Korean Journal of pain. , 34 (3):1-9.
Faculty: Science

Abstract: Background Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel implicated in pain sensation in response to heat, protons, and capsaicin (CAPS). It is well established that TRPV1 is involved in mechanical allodynia. This study investigates the effect of Ononis spinosa (Fabaceae) in CAPS-induced mechanical allodynia and its mechanism of action. Methods Mechanical allodynia was induced by the intraplantar (ipl) injection of 40 µg CAPS into the left hind paw of male Wistar rats. Animals received an ipl injection of 100 µg O. spinosa methanolic leaf extract or 2.5% diclofenac sodium 20 minutes before CAPS injection. Paw withdrawal threshold (PWT) was measured using von Frey filament 30, 90, and 150 minutes after CAPS injection. A molecular docking tool, AutoDock 4.2, was used to study the binding energies and intermolecular interactions between O. spinosa constituents and TRPV1 receptor. Results The ipsilateral ipl injection of O. spinosa before CAPS injection increased PWT in rats at all time points. O. spinosa decreased mechanical allodynia by 5.35-fold compared to a 3.59-fold decrease produced by diclofenac sodium. The ipsilateral pretreatment with TRPV1 antagonist (300 µg 4-[3-Chloro-2-pyridinyl]-N-[4-[1,1-dimethylethyl] phenyl]-1-piperazinecarboxamide [BCTC]) as well as the β2-adrenoreceptor antagonist (150 µg butoxamine) attenuated the action of O. spinosa. Depending on molecular docking results, the activity of the extract could be attributed to the bindings of campesterol, stigmasterol, and ononin compounds to TRPV1. Conclusions O. spinosa alleviated CAPS-induced mechanical allodynia through 2 mechanisms the direct modulation of TRPV1 and the involvement of β2 adrenoreceptor signaling.

Keywords: Butoxamine, Capsaicin, Fabaceae, Hyperalgesia, Molecular Docking Simulation, Neuralgia, Ononis, Pain, Stigmasterol, TRPV Cation Channels

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Research Title: TRP channels in COVID-19 disease: Potential targets for prevention and treatment.
Author: Sahar Majdi Jaffal, Published Year: 2021
Chemico-Biological Interactions. , 245 (109567): 1-11.
Faculty: Science

Abstract: Coronavirus disease 2019 [COVID-19] is a global health threat caused by severe acute respiratory syndrome coronavirus 2 [SARS-CoV2] that requires two proteins for entry: angiotensin-converting enzyme 2 [ACE2] and -membrane protease serine 2 [TMPRSS2]. Many patients complain from pneumonia, cough, fever, and gastrointestinal (GI) problems. Notably, different TRP channels are expressed in various tissues infected by SARS-CoV-2. TRP channels are cation channels that show a common architecture with high permeability to calcium [Ca2+] in most sub-families. Literature review shed light on the possible role of TRP channels in COVID-19 disease. TRP channels may take part in inflammation, pain, fever, anosmia, ageusia, respiratory, cardiovascular, GI and neurological complications related to COVID-19. Also, TRP channels could be the targets for many active compounds that showed effectiveness against SARS-CoV-2. Desensitization or blocking TRP channels by antibodies, aptamers, small molecules or venoms can be an option for COVID-19 prevention and future treatment. This review provides insights into the involvement of TRP channels in different symptoms and mechanisms of SARS-CoV-2 , potential treatments targeting these channels and highlights missing gaps in literature.

Keywords: COVID-19; Prevention; SARS-CoV-2; Symptoms; TRP channels; Treatment.

50
Research Title: Anti-inflammatory and antipyretic potential of Arbutus andrachne L. methanolic leaf extract in rats.
Author: Sahar Majdi Jaffal, Published Year: 2021
Asian Pacific Journal of Tropical Biomedicine, 11(11): 491-499.
Faculty: Science

Abstract: Abstract Objective: To explore the anti-inflammatory and antipyretic effects of methanolic leaf extract from Arbutus andrachne and its mechanism of action. Methods: Paw edema was induced by intraplantar (i.pl.) injection of λ-carrageenan (1% w/v, 100 μL/paw) while pyrexia was evoked by intraperitoneal (i.p.) injection of 20?ker's yeast (20 mL/kg body wt) in male Wistar rats. The anti-inflammatory and antipyretic effects of Arbutus andrachne methanolic leaf extract were explored by injecting rats with different doses of the plant extract (150, 300, and 600 mg/kg body wt, i.p.). Selective antagonists for transient receptor potential vanilloid-1 (TRPV1), cannabinoid receptor 1 (CB1), and alpha-2 adrenergic receptor (a2-AR) were used to unravel the extracts’ mechanism of action. Blood samples were collected from the heart of rats to measure the levels of interleukin-6 (IL-6) and prostaglandin E2 (PGE2) by enzyme-linked immunosorbent assay. Results: The extract exhibited potent anti-inflammatory activity by decreasing paw thickness and IL-6 levels. In addition, yeast-evoked pyrexia was attenuated by the extract treatment via TRPV1 and CB1 receptors and a reduction in PGE2 levels. No significant effects were found for a2-AR. Moreover, the rats that received the plant extract demonstrated similar responses to the positive control group. Conclusions: Arbutus andrachne can be a good candidate for treating inflammation and pyrexia and should be further investigated.

Keywords: Arbutus andrachne; Anti-inflammatory; Antipyretic; PGE2; IL-6; TRPV1; CB1; a2-ARs