1141 |
Research Title: Microbiology, biotechnology and biosafety.
Author: Sameer Masoud, Published Year: 2004
Faculty: Science
Abstract: CONSERVATION AND SUSTAINABLE USE
OF BIOLOGICAL DIVERSITY IN
JORDAN
First National Report
of The Hashemite Kingdom of Jordan on the Implementation of Article
6 of the Convention on Biological Diversity
http://www.philadelphia.edu.jo/academics/smasoud/uploads/R4.pdf
Keywords: biodiversity
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1142 |
Research Title: Overexpression of L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase in tobacco cell suspension cultures.
Author: Sameer Masoud, Published Year: 1999
Faculty: Science
Abstract: Overexpression of L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase in tobacco cell suspension cultures.
In: Plant Biotechnology and in vitro Biology in the 21st Century.
A. Altman et al. (eds), Kluwer Academic Publishers,
The Netherlands. PP 297-301.
Keywords: plant resistance
|
1143 |
Research Title: Improvement of natural defense responses. In: Engineering Plants for Commercial Products and Applications
Author: Sameer Masoud, Published Year: 1996
Annals of the New York Academy of Sciences 792:126-139, 792
Faculty: Science
Abstract: Improvement of natural defense responses. In: Engineering Plants for Commercial Products and Applications
Keywords: biotechnology
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1144 |
Research Title: Metabolic engineering: Prospect for crop improvement through genetic manipulation of phenylpropanoid biosynthesis and defense responses.
Author: Sameer Masoud, Published Year: 1999
Gene, 179
Faculty: Science
Abstract: In leguminous plants such as the forage legume alfalfa, products of
the phenylpropanoid pathway of secondary metabolism are involved
in interactions with beneficial microorganisms (flavonoid inducers of
the Rhizobium symbiosis), and in defense against pathogens
(isoflavonoid phytoalexins). In addition, the phenylpropane polymer
lignin is a major structural component of secondary vascular tissue
and fibers in higher plants. The recent isolation of genes encoding
key enzymes of the various phenylpropanoid branch pathways opens
up the possibility of engineering important crop plants such as alfalfa
for: (a) improved forage digestibility, by modification of lignin
composition and/or content; (b) increased or broader-spectrum
disease resistance, by introducing novel phytoalexins or structural
variants of the naturally occurring phytoalexins, or by modifying
expression of transcriptional regulators of phytoalexin pathways; and
(c) enhanced nodulation efficiency, by engineering over-production of
flavonoid nod gene inducers. The basic biochemistry and molecular
biology underlying these strategies is briefly reviewed, and recent
progress with transgenic plants summarized. The potential
importance of metabolic compartmentation for attempts to engineer
phenylpropanoid biosynthetic pathways is also discussed. Overexpression of an alfalfa glucanase-encoding gene confers significant
protection against Phytophthora in alfalfa, possibly via indirect effects
on phenylpropanoid metabolism.
Keywords: phytoalexins
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1145 |
Research Title: Meat and Organs Quality of Broiler Chickens Fed Diet Contaminated with B1 Aflatoxin
Author: Sameer Masoud, Published Year: 2014
Global Veterinaria , 12
Faculty: Science
Abstract: The levels of aflatoxin B1 (AFB1) in breast, leg, liver, kidney and gizzard and in litter were studied in
broilers chicks maintained for 6 weeks on AFB1 contaminated diets of 0.0(group 1,control group), 384.5 µg/kg
AFB1 (group2,treatment 1), or 128.9 µg/kg AFB1(group3,treatment2). The highest AFB1 of 1.2 µg/kg was at the
third week in liver tissues and 0.8 µg/kg in chicken legs fed diet contaminated with 374.53 ppb AFB1. Breast and
gizzard showed lower AFB1 concentrations of 0.5 and 0.8 µg/kg, respectively, than treatment 1 at the end of
the third week. The residual level of AFB1 were increased in liver and kidney of 2.1 and 1.9 µg/kg AFB1 at wk
6 and chickens breast and leg AFB1 levels also affected and increased to 0.93 and 1.64 µg/kg, respectively.
Keywords: Broiler Aflatoxin B1 Organs Litter
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1146 |
Research Title: DNA extraction and PCR-based diagnosis of the root-knot nematodes (Meloidogyne species and races) of Jordan.
Author: Sameer Masoud, Published Year: 2010
Jordan Journal of Agricultural Sciences, 6
Faculty: Science
Abstract: Three Meloidogyne spp. of the root-knot nematodes were recently surveyed in
Jordan and identified as M. javanica, M. incognita (race 1 and 2), M. arenaria (race 2),
based on a combination of several diagnostic methods. Several methods of genomic DNA
extraction from nematode eggs, single or many 2nd stage juveniles and females were
evaluated. For DNA fingerprinting, sequence characterized amplified regions (SCAR)
and random amplified polymorphic DNA (RAPD) based polymerase chain reaction
(PCR) assays were used. Among the tested DNA extraction methods, miniprep method
was the most efficient, cost and time effective for SCAR-PCR. Methods used for DNA
extraction from single juveniles or females were more suitable for RAPD than SCARPCR. Typical DNA products of 670, 420, or 1200 bp in size were specifically amplified
by SCAR-PCR when DNA extracts of M. javanica, M. arenaria (race 2), or M. incognita
(race 1 or 2), respectively, were used as template DNA. Accordingly, Meloidogyne
species in Jordan could be most reliably identified using SCAR based PCR assay. The
primer PA-01 produced RAPD patterns with clear bands that clearly distinguished one
species from the others and so allowed the identification of the three Meloidogyne
species. Molecular techniques for the identification of Meloidogyne spp. will be
particularly useful in cases of mixed populations of the three species and for reliable
quarantine tests
Keywords: DNA fingerprint
|
1147 |
Research Title: Assessment of phenotypic diversity among Jordanian barley landraces (Hordeum vulgare L.).
Author: Sameer Masoud, Published Year: 2007
Biotechnology, 6
Faculty: Science
Abstract: Assessment of phenotypic diversity among Jordanian barley landraces (Hordeum vulgare L.).
Keywords: RAPD PCR
|
1148 |
Research Title: Serologic and molecular characterization of Pseudomonas aeruginosa Jordanian clinical isolates compared with the strains of International Antigenic Typing Scheme.
Author: Sameer Masoud, Published Year: 2007
Diagnostic Microbiology and Infectious Disease, 58
Faculty: Science
Abstract: One hundred clinical isolates of Pseudomonas aeruginosa were serologically classified
into 7 Jordanian serotypes (labeled JO1–JO7) Odeh, 2002, M.Sc. thesis, University of
Jordan). Using the slide agglutination test, 4 of them (JO4, JO5, JO6, and JO7) were
serologically matched with the International Antigenic Typing Scheme (IATS) strains
(IATS 20, IATS 10, IATS 6, and IATS 11). One serotype (JO1) showed a weak crossreaction with IATS 1. The remaining 2 local serotypes (JO2 and JO3) did not react with
any of the 20 IATS strains. Serologic analysis data showed to a certain extent correlations
with molecular data using genetic clustering and similarity indices generated by random
amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). Each of the 4
identified local serotypes formed a cluster with its serologically matched IATS strain
with relatively high average similarity indices, whereas lower average similarity index
was observed between IATS 1 and JO1, in consistence with the weak serologic reaction
using the slide agglutination test. On the other hand, the 2 nontypeable serotypes (JO2
and JO3) formed 2 separate clusters that could not be matched to any of the IATS strain.
Phenotypic and genotypic analyses suggest that JO2 and JO3, and possibly JO1, can be
new serotypes of P. aeruginosa. RAPD-PCR was also used to study the relative
relatedness among the 20 IATS strains. The IATS strains formed 2 main clusters. Half of
the IATS strains formed one main cluster that included IATS 11–20. The remaining
IATS strains (8 strains) formed the second main cluster, with the exception of IATS 4
and 9, where each formed a separate cluster.
Keywords: random primers, PCR
|
1149 |
Research Title: Assessment of genetic variation among Jordanian barley landraces (Hordeum vulgare L.) as reveled by molecular markers.
Author: Sameer Masoud, Published Year: 2007
Faculty: Science
Abstract: Assessment of genetic variation among Jordanian barley landraces (Hordeum vulgare L.) as reveled by molecular markers.
http://www.idosi.org/aejaes/aejaes2(1).htm
Keywords: RAPD PCR
|
1150 |
Research Title: A comparison among diagnostic means used to identify root-knot nematodes (Meloidogyne species and races) from Jordan.
Author: Sameer Masoud, Published Year: 2006
Pak. J. Nematol., 24
Faculty: Science
Abstract: A comparison among diagnostic means used to identify root-knot nematodes (Meloidogyne species and races) from Jordan.
Keywords: pcr
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